Fig. 5: Profiling the mural and polar TE sublineages in the guinea pig embryo.
From: The guinea pig serves as an alternative model to study human preimplantation development
a,b, Two-dimensional UMAP of guinea pig TE cells (from 29 embryos) stratified by sublineage (a) and developmental stage (b), with colours corresponding to sublineages and the developmental stages, respectively. c, Heatmap representation displaying the DEGs between TE sublineages. Genes that were also significantly differentially expressed between human mural and polar TE cells are labelled on the right. Red and black colours indicate the same and opposite expression patterns, respectively. d, Feature plot displaying the expression of NR2F2 in guinea pig TE cells (from 29 embryos). e, Immunofluorescence image of a representative in vivo guinea pig embryo at the late blastocyst stage labelled with Hoechst (nucleus), SOX2+ (EPI), NR2F2+ GATA3+ (mural TE) and NR2F2− GATA3+ (polar TE). The dotted oval outlines the EPI cells within the embryo. f, Quantification of cells per embryo expressing SOX2+ (EPI), NR2F2+ GATA3+ (mural TE) and NR2F2− GATA3+ (polar TE) in guinea pig in vivo late blastocysts. Cells with only Hoechst staining represent the PE. Bar graphs present the mean ± s.e.m. of n = 4 replicates. Scale bar, 20 µm.
