Fig. 6: Implantation pole analysis of guinea pig, human and mouse embryos.
From: The guinea pig serves as an alternative model to study human preimplantation development
a, Dot plot illustrating the enriched KEGG pathways for DEGs between mural and polar TE cells in guinea pig (*P < 0.05, one-sided, calculated by permutation test). Colour and size indicate the significance and number of DEGs in each pathway. b, Schematic of the JAK-STAT inhibition treatment protocol. c, Representative immunofluorescence images of SOX2 (EPI, yellow), NR2F2 (mural TE, cyan) and Hoechst nuclear staining (blue) in control- (DMSO) and JAK-STAT inhibitor- (10 µM AZD1480) treated guinea pig embryos. d, Scatter plot showing the total number of cells per embryo (Hoechst stained) and the number of cells per embryo for the respective lineage markers analysed in control- (n = 6) and AZD1480- (n = 6) treated embryos. The P value is stated in the figure (two-tailed Mann–Whitney test). e, Representative immunofluorescence images of SOX2 (yellow), NR2F2 (cyan), RXRα (red) and Hoechst nuclear staining (blue) in guinea pig embryos at mid and late blastocyst (n = 4). f, Z-stack slice from a representative immunofluorescence image of SOX2 (yellow), NR2F2 (cyan), RXRα (red) and Hoechst nuclear staining (blue) in human embryos at late blastocyst (n = 4). g, Immunofluorescence representation of a late mouse blastocyst analysing Sox2 (yellow), Nr2f2 (cyan) and Cdx2 (grey); the total cells are visualized by Hoechst (blue) (n = 5). h, Immunofluorescence representation of a mouse late blastocyst (90-cell embryo, E4.5) analysing Sox2 (yellow) and Rxrα (red), with the total number of cells visualized by Hoechst (blue) (n = 5). i, UMAP analyses showing the developmental progression of mouse cells from the eight-cell stage to late blastocyst (left) and Rxrα expression (right) during preimplantation development. Data from ref. 39. Scale bars, 20 µm.
