Extended Data Fig. 1: Preimplantation development features of guinea pig and mouse embryos.
From: The guinea pig serves as an alternative model to study human preimplantation development
a, Representative immunofluorescence images of EPI (SOX2), PE (SOX17), and TE (CDX2) associated markers during precavitation. The dotted cyan circle indicates an emerging cell with CDX2+ expression only, observed in only 2 of 6 embryos presented at this stage. b, Scatter plot depicting the total number of CDX2+ cells per embryo relative to the total number of cells per embryo (Hoechst) (n = 6 embryos collected at E4.5-4.75), along with linear regression illustrating the variability in CDX2 expression at this stage. c, Representative immunofluorescence image of EPI (SOX2), PE (SOX17), and TE (CDX2) associated markers during cavitation (E4.75-5). The magenta dotted circle indicates two emerging cells with SOX17+ expression, observed in only 2 of 4 embryos. d and e, Quantification of immunofluorescence images displayed in Fig. 1c and f. Bar graphs of the number of cells per mouse embryo for (d) Sox2, Sox17 and Cdx2 only positive cells as well as their co-expression at different developmental stages (compacted morula (n = 4), precavitation (n = 4), early (n = 3) mid (n = 3) and late blastocysts (n = 4)) and (e) Sox2, Gata6 and Gata3 only positive cells as well as their co-expression at different developmental stages (compacted morula (n = 5), precavitation (n = 4), early (n = 3) mid (n = 4) and late blastocysts (n = 4). Scale bars: 20 µm.
