Extended Data Fig. 2: Overview of cryo-FIB milling and reconstructed tomograms.
From: Nuclear pores safeguard the integrity of the nuclear envelope
(a) Representative cryo-FIB milled lamella of the wild-type mES cells. FIB view of an mES cell on a cryo-EM grid before cryo-FIB milling (top) and SEM view of the same cell after cryo-FIB milling (bottom) are shown. (b) Cryo-TEM overview of the lamella shown in (a), rotated by 180°. (c, d) Representative cryo-FIB milled lamella of the wild-type neural progenitor cells, shown as in (a) and (b). 41 and 27 lamellae of similar quality were prepared for the wild-type mES and neural progenitor datasets, respectively. (e, f) Representative slices through reconstructed tomograms of the wild-type mES cells (e) and the wild-type neural progenitor cells (f). Top views of the NPCs are indicated by white arrowheads. (g, h) Representative tomographic slices showing the side views of the nuclear envelope of the wild-type mES cells (g) and the wild-type neural progenitor cells (h). Side views of the NPCs are indicated by white arrowheads. For (e - h), the total numbers of acquired tomograms are provided in Supplementary Table 1. (i) Quantification of the nuclear envelope thickness. The thickness was measured manually at more than six representative positions within the tomograms, and the averaged values were plotted. Measurement was performed for randomly selected 15 tomograms with clear side views of the nuclear envelope. Bars denote the means and standard deviations. Kruskal-Wallis rank sum test followed by Dunn’s multiple comparisons test. Source numerical data are available in source data.
