Fig. 3: NPCs with non-canonical symmetries are present in Nup133−/− mES cells.
From: Nuclear pores safeguard the integrity of the nuclear envelope
a–f, Representative slices from reconstructed tomograms of the Nup133−/− mES cells, showing top views of seven-fold (a,c), eight-fold (a,b,d,f) and nine-fold (d,e) symmetric NPCs. In a,d, the eight-fold symmetric NPCs are indicated by light blue arrowheads, and NPCs with seven-fold (c) and nine-fold (e) symmetric architectures are indicated by green and purple arrowheads, respectively. The top views in a and d are shown as enlarged views in b, c, e and f, with protomers numbered. Particle numbers are provided in Supplementary Table 1. Scale bars, 50 nm (b,c,e,f). g, Pie chart of the particle distribution after reference-based classification. Particles with ambiguous class assignment are indicated in light grey, and particles discarded before classification are in dark grey. h–j, Composite cryo-EM maps of the seven-fold (h), eight-fold (i) and nine-fold (j) symmetric NPCs, shown as a cutaway view (top) and a cytoplasmic view (bottom). Note that the CR and NR of the seven-fold symmetric NPC, as well as the NR of the nine-fold symmetric NPC did not yield interpretable subtomogram averages and are not included in the composite cryo-EM maps in h and j.
