Fig. 6: Over-stretched NPCs are present in Nup133−/− neural progenitor cells.
From: Nuclear pores safeguard the integrity of the nuclear envelope
a, Hypothetical model of NPC diameter changes during differentiation: (i) in wild-type cells, the increased nuclear envelope tension during early neuronal differentiation is homogeneously propagated along the nuclear envelope, leading to overall dilation of the NPCs; (ii) in Nup133−/− cells, the increased nuclear envelope tension causes over-stretching of NPCs with impaired structural robustness, leading to release of the nuclear envelope tension and overall constriction of the NPCs. The nuclear envelope is coloured in light blue, and the CR, IR and NR of the NPC are coloured in yellow, blue and pink, respectively. Red and black arrows depict nuclear envelope membrane tension and the motion of NPC scaffolds. b, Representative example of the over-stretched NPCs observed in the Nup133−/− neural progenitor dataset: slices from the reconstructed tomograms showing the side views (left) and top views (middle) of the over-stretched NPCs, together with pseudo-composite maps generated from the results of the TM analysis (right). CRs, IRs and NRs in the pseudo-composite maps are coloured as in Fig. 3a and are shown with the segmented membrane (light blue). The site of CR detachment from the outer nuclear membrane is highlighted with a grey arrowhead. All analysed over-stretched NPCs are shown in Extended Data Fig. 7. c, Quantification of the over-stretched NPCs in the four datasets (wild-type mES and neural progenitor cells; Nup133−/− mES and neural progenitor cells). The number of analysed NPCs and the percentage of over-stretched NPCs (diameter > 135 nm) are indicated on top of each bar. d, Histogram showing the distribution of the measured NPC diameters depicted in c. Note that the overall diameter distribution is consistent with the results of subtomogram average-based measurements in Fig. 1d.
