Fig. 7: Accumulation of DNA damage is observed in Nup133−/− neuronal precursors.
From: Nuclear pores safeguard the integrity of the nuclear envelope
a, Representative immunofluorescence staining images of neuronal precursors with γ-H2AX and 53BP1 foci 24 h after neuronal differentiation induction. Maximum projection images are shown. Examples of nuclei with ≥5 detected foci are indicated with white arrowheads and highlighted in the insets. Scale bars, 10 μm. b,c, Mean percentage of nuclei with ≥5 γ-H2AX (b) and 53BP1 (c) foci in wild-type and Nup133−/− neuronal precursors. Data are from three biological replicates, and at least 330 nuclei were analysed for each replicate. Each data point represents the percentage calculated from the individual dataset. Error bars denote s.d. Two-tailed Student’s t-test. d, Representative x–z orthogonal views of nuclei from neuronal precursors with γ-H2AX and 53BP1 foci. The nuclei highlighted with white arrowheads in a are shown. Scale bars, 10 µm. e, Representative immunofluorescence staining images of neuronal precursors with nuclear YAP 48 h after neuronal differentiation induction. Maximum projection images are shown together with x–z orthogonal views of the nuclei highlighted with white arrowheads. Scale bars, 10 μm. f–h, Mean percentage of nuclei with nuclear YAP localization in neuronal precursors at 2 h (f), 24 h (g) and 48 h (h) time points after neuronal differentiation induction. Data are from two (f) or four (g,h) biological replicates, and at least 340 nuclei were analysed for each replicate. In g and h, error bars denote s.d. Two-tailed Student’s t-test. i–k, Mean percentage of nuclei with flatness index below 0.8 in neuronal precursors at 2 h (i), 24 h (j) and 48 h (k) after neuronal differentiation induction. Data are from two (i) or four (j,k) biological replicates, and at least 340 nuclei were analysed for each replicate. In j and k, error bars denote s.d. Two-tailed Student’s t-test.
