Extended Data Fig. 6: Impact of either LLOMe or OSW1 on the localization of the ATG2C domain or the PH domain of VPS13C. | Nature Cell Biology

Extended Data Fig. 6: Impact of either LLOMe or OSW1 on the localization of the ATG2C domain or the PH domain of VPS13C.

From: The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage

Extended Data Fig. 6

a, Live fluorescence images of RPE1 cells expressing mCherry-PH and LAMP1-GFP before and after 1 mM LLOMe addition. b,c, Live fluorescence images of RPE1 cells expressing mCherry-ATG2CVPS13C and GFP-OSBP before and after addition of 20 nM OSW1 (b) or 1 mM LLOMe (c). Black dots visible before LLOMe are lipid droplets that move slightly in position after LLOMe. Individual channel images are shown as inverted greys. Experiments were repeated three times with similar results.

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