Extended Data Fig. 8: Depletion of VPS13C does not affect the lysosomal recruitment of OSBP and IST1. | Nature Cell Biology

Extended Data Fig. 8: Depletion of VPS13C does not affect the lysosomal recruitment of OSBP and IST1.

From: The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage

Extended Data Fig. 8

a, Anti-VPS13C western blot of whole cell lysates from WT or VPS13C knockout A549 cells. Tubulin was used as a loading control. b,c, Live fluorescence images of the WT and VPS13C-KO A549 cells expressing mCherry-OSBP and LAMP1-GFP (b) or IST1-Apple and LAMP1-GFP (c) and stained with LysoView640 before and after 1 mM LLOMe treatment. Individual channel images are shown as inverted greys. Experiments were repeated three times with similar results. Unprocessed blots are provided.

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