Extended Data Fig. 9: Depletion of VPS13C causes disruption of lysosome homeostasis. | Nature Cell Biology

Extended Data Fig. 9: Depletion of VPS13C causes disruption of lysosome homeostasis.

From: The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage

Extended Data Fig. 9

a, Anti-LAMP1 immunofluorescence of WT or VPS13C-KO A549 cells. b, Live fluorescence images of WT or VPS13C-KO A549 cells incubated with the pH sensitive LysoView633 showing lower fluorescence (higher pH) of the KO cells. c, Time-series of live fluorescence images of WT or VPS13C-KO Hela cells expressing Gal3-GFP showing recruitment of Gal3 to damaged lysosomes upon 1 mM LLOMe treatment. Quantification of the intensity of the Gal3-GFP punctate fluorescence per cell after addition of 1 mM LLOMe is shown on the right. n = 66 cells (WT), n = 81 cells (VPS13C-KO) collected from three biological replicates. Shaded areas, mean ± SD. Individual channel images are shown as inverted greys. Experiments were repeated three times with similar results. Numerical data are provided.

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