Fig. 2: VPS13C functions at the ER–endolysosome membrane contact sites.
a, Cartoon depicting VPS13C localized at the ER–endolysosome membrane contact sites. b, High-magnification time-series images of VPS13CmClover-Flp-In cells co-expressing exogenous mCh–VAPB following treatment with 1 mM LLOMe. c, Fluorescence images of live VPS13CmClover-Flp-In cells showing VPS13CmClover localization in control (siControl, control siRNA) and Rab7-knockdown (siRab7, siRNA to Rab7) cells before and after treatment with 1 mM LLOMe (top). Fluorescence intensity of VPS13CmClover puncta signals per cell before and after LLOMe treatment (bottom). Error bars represent the s.d.; n = 44 siControl and 60 siRab7 cells collected from three biological replicates. Data were compared using a two-sided Student’s t-test; NS, not significant (P = 0.7437) and ****P < 0.0001. d, Fluorescence images of live WT and Rab7-KO HeLa cells, expressing VPS13CHalo and labelled with LysoView 640 (a luminal marker of acidic lysosomes), before and after treatment with 1 mM LLOMe. Black lines represent cell outlines. Magnified views of the boxed regions in the VPS13CHalo channel are shown (bottom). b,d, The experiments were repeated three times with similar results. All the individual channel images in this figure are shown as inverted greys. Numerical source data are provided.
