Fig. 6: Loss of VPS13C increases the fragility of lysosomes.
a,b, Intensity of punctate LAMP1 immunofluorescence (a) and LysoView 633 fluorescence (a pH-sensitive lysosome probe; b) per WT and VPS13C-KO A549 cell. a, n = 240 cells per group. b, n = 84 WT and 76 VPS13C-KO cells. c,d, Gal3-to-LAMP1 ratio (c) determined from the images in d. c, Normalized fluorescence relative to WT cells treated with LLOMe for 25 min. NS, P = 0.2886; n = 32 (WT, 0 min; WT, 5 min; and VPS13C-KO, 5 min), 33 (VPS13C-KO, 0 min), 34 (WT, 15 min), 31 (VPS13C-KO, 15 min), 39 (WT, 25 min) and 35 (VPS13C-KO, 25 min) images analysing >300 cells. d, The images depict fluorescence microscopy of WT and VPS13C-KO A549 cells immunolabelled with antibodies to Gal3 and LAMP1 following treatment with or without 1 mM LLOMe for the indicated times. Fluorescence images from a representative experiment. a–c, Three biological replicates. Data are the mean ± s.d. Data were compared using a two-sided Student’s t-test; ****P < 0.0001; NS, not significant. Numerical data are provided.
