Fig. 7: Relationship between VPS13C and LRRK2 recruitment to damaged lysosomes.
a, Schematic illustration of the CASM pathway and experimental manipulations that trigger (blue) or inhibit (red) it. b, Summary of agents tested and their impact on the recruitment of VPS13C to lysosomes. c, Fluorescence images of live VPS13CmClover-Flp-In cells, labelled with LysoView 633, before and after Salip treatment. Dashed lines represent cell outlines. d, Fluorescence images of live VPS13CmClover-Flp-In cells with exogenous mCh–SopF co-expression before and after treatment with 1 mM LLOMe. Cells not expressing mCh–SopF are outlined by dashed lines. e, Time-series fluorescence images of live HeLa cells co-expressing VPS13CHalo and GFP–LRRK2 showing recruitment of VPS13C and LRRK2 to lysosomes following treatment with 1 mM LLOMe (left). Individual channel images are shown as inverted greys. Relative punctate fluorescence intensity of VPS13CHalo and GFP–LRRK2 per cell after LLOMe treatment in a single experiment (right). Mean ± s.d. represented by the solid line and shaded area, respectively; n = 15 VPS13C and 11 LRRK2 cells were analysed. c–e, The experiments were repeated three times with similar results. Further examples in Extended Data Fig. 10b,c. Numerical source data are provided.
