Extended Data Fig. 2: LLOMe-induced lysosomal recruitment of VPS13C precedes of ESCRT-III. | Nature Cell Biology

Extended Data Fig. 2: LLOMe-induced lysosomal recruitment of VPS13C precedes of ESCRT-III.

From: The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage

Extended Data Fig. 2

a, Time-series of live fluorescence images of VPS13C^mClover-Flp-In cells also co-expressing IST1-Apple, upon 1 mM LLOMe treatment. Note the delayed recruitment of IST1 to lysosomes relative to VPS13C. Individual channel images are shown as inverted greys. The experiment was repeated three times with similar results. b, Live fluorescence images of VPS13C^mClover-Flp-In cells also co-expressing IST1-Apple, 20 minutes after addition of 1 mM LLOMe. Cells were pre-incubated for 1 hr with or without the calcium chelator BAPTA (20 μM). Recruitment of IST1, but not of VPS13C, is inhibited by BAPTA. The experiment was repeated three times with similar results.

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