Fig. 6: Enhanced cell cycle progression and leptomeningeal colonization in metastatic tumour cells with active BMP signalling.
a, Violin plots showing cell cycle progression gene expression in Id1− and Id1+ LPT-MET tumour cells from the scRNA-seq data. Two-tailed Student’s t-test was performed. *P < 2.22 × 10−16. b, Whole-mount Ptch-SB spinal leptomeninges with metastasis fluorescently labelled with GFP, ID1 and Ki67 with the proportion of ID1 and Ki67 double-positive cells quantified per field of view (n = 5 images; the centre line represents the mean, and error bars represent the standard deviation; scale bars, 120 µm). c, BLI 38 days after NSG mice were implanted with D458 Luc-ZsGreen tumour cells expressing control and BMPR1A-CA into the lateral ventricle (cohort of n = 5 mice per group are shown). d, Total flux (photons s−1) quantifications of D458 Luc-ZsGreen control and BMPR1A-CA (n = 10 mice per group). e, The proportion of GFP-positive area on the surface of the brain, spinal cord and peripheral nerves of D458 Luc-ZsGreen control and BMPR1A-CA (n = 10 mice per group). P values were generated using two-tailed unpaired t-test with Welch’s correction, the centre line represents the mean, and error bars represent the standard deviation for d and e. f, Kaplan–Meier survival analysis from NSG mice implanted with D458 Luc-ZsGreen control (n = 9 mice) and BMPR1A-CA (n = 10 mice) into the lateral ventricle (log-rank (Mantel–Cox) test was performed).
