Extended Data Fig. 2: Effects of stress on mouse allograft tumor progression. | Nature Cell Biology

Extended Data Fig. 2: Effects of stress on mouse allograft tumor progression.

From: Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA

Extended Data Fig. 2

a and b, Restraint stress (a) and CUS (b) significantly enhance the growth of KPC cell-derived allograft in C57BL/6J mice. Left panel shows bioluminescence imaging of tumors and right panel is quantitative statistics (n = 5 mice). c and d, Restraint stress (c) and CUS (d) enhance tumor cell proliferation indicated by Ki67 in orthotopic tumor tissue (n = 5 mice). e–h, Nociception stress significantly promotes tumor nerve innervation (e, β3-tubulin+) including sympathetic nerve (f, TH+), sensory nerve (g, Trpv1+) but not parasympathetic nerve (h, VAchT+) innervation in mouse allograft tumors. Left panel shows immunofluorescence images and right panel shows quantitative statistics (n = 5 mice). i–l, Restraint stress significantly promotes tumor nerve innervation (i, β3-tubulin+) including sympathetic nerve (j, TH+), sensory nerve (k, Trpv1+) but not parasympathetic nerve (l, VAchT+) innervation in mouse allograft tumors (n = 5 mice). m–p, CUS significantly promotes tumor nerve innervation (m, β3-tubulin+) including sympathetic nerve (n, TH+), sensory nerve (o, Trpv1+) but not parasympathetic nerve (p, VAchT+) innervation in mouse allograft tumors (n = 5 mice). q–s, Nociception stress (q), restraint stress (r) and CUS (s) cause significant increment of plasma noradrenaline (Left) and corticosterone (Right) in orthotopic PDAC mice (n = 5 mice). t and u, Open-field test (t) and Light-dark box test (u) show behavioral change of orthotopic PDAC mice after exposure to nociception stress (n = 5 mice). v and w, Open-field test (v) and Light-dark box test (w) show behavioral change of orthotopic PDAC mice after exposure to restraint stress (n = 5 mice). x and y, Open-field test (x) and Light-dark box test (y) show behavioral change of orthotopic PDAC mice after exposure to CUS (n = 5 mice). Scale bars, 200 μm (c–p). All bar chart data are means ± s.e.m. from 5 mice, with P value by two-tailed Student’s t-test in a, b, d–y and by Wilcoxon rank-sum test in c.

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