Extended Data Fig. 8: SPIN1 participates in DSB-induced transcriptional repression in cis.
From: Transcriptional repression facilitates RNA:DNA hybrid accumulation at DNA double-strand breaks
(a) Genomic tracks (hg19) of SPIN1, H3K4me3 and RNAPII (405 A) ChIP-seq as well as RNA-seq before DSB induction showing the similarities between the signals detected. (b) Average profile and heatmap of SPIN1 ChIP-seq signal on all genes −/+ 2 kb (normalized read count). (c) Metagene profiles of ChIP-seq signals for H3K4me3 (left panel) and SPIN1 (right panel) on genes classified into three classes according to their RNAPII levels (high, mid or low) before DSB induction. (d) Levels of SPIN1 and Myosin detected by Western blot after siRNA-mediated depletion of SPIN1. A representative experiment is shown (out of n = 4). (e) Levels of SPIN1, H3K4me3 and Tubulin detected by Western blot after treatment with MS31 (n = 1). (f) ChIP–qPCR efficiency (%input) of either H3K4me3 (left panel) or SPIN1 (right panel) at a control intergenic locus (Ctrl1) and at the promoter of CDKN1A and GAPDH in the presence or absence of MS31 treatment (mean and s.e.m. of technical replicates (n = 4)). (g) Average profiles and heatmaps of the differential enrichment of qDRIP-seq signal after depletion of SPIN1 (siSPIN1 +DSB (-) siCtrl +DSB) on hybrid-positive and -negative DSBs. Source numerical data and unprocessed blots are available in source data.
