Extended Data Fig. 9: Factors participating in DSB-induced transcriptional repression participate in HR and cell survival.
From: Transcriptional repression facilitates RNA:DNA hybrid accumulation at DNA double-strand breaks
(a) Number of BRCA1 or RAD51 foci in the presence or absence of Etoposide (Eto) and/or MS31. Red line: median. P values, paired two-sided nonparametric Wilcoxon tests. (b) Clonogenic assay in MS31-treated DIvA-AID cells after DSB induction (+DSB). A representative experiment is shown on the left panel. Mean and s.e.m. for n = 3 biological replicates are shown on the right panel. P values, paired two-sided t-test. (c) Percentage of cell survival upon treatment with different doses of Etoposide treatment (indicated on the x axis) and in the presence or absence of MS31. (d) Clonogenic assay in control (Ctrl) or NELF-E siRNA-treated DIvA-AID cells in -DSB and +DSB conditions and after IAA treatment allowing DSB repair (+DSB +repair). Mean and s.e.m. for n = 4 biological replicates are shown. P values, paired two-sided t-tests. (e) same as in (d) for control (Ctrl), SPIN1 or SPIN1 + SETX siRNA-treated DIvA-AID cells, n = 3 biological replicates. P values, paired two-sided t-test. Source numerical data are available in source data.
