Extended Data Fig. 5: LMNA KO dHL60 cells have a nuclear morphology closer to PMNs relative to SCR dHL60 cells.
From: Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation
a. Western blot images representative of three independent experiments, showing the levels of lamin A/C, lamin B1, lamin B2, and LBR in SCR, LMNA KO, and LMNA/LBR KO dHL60 cell lysates. GAPDH is loading control. The molecular weights (kilodaltons, kDa) are indicated on left. b. Airyscan microscopy images of PMNs, SCR, LMNA KO, and LMNA/LBR KO dHL60 cells migrating towards fMLF, fixed and stained for LBR (magenta, nuclear envelope) and Hoechst (cyan, nucleus). Presented “sum of slices” projections are representative of three independent experiments. Dashed white/black outlines indicate cell shape. Scale is 5 μm. c-f. Scatter dot plots showing (c) NE to cytoplasm LBR intensity ratio, (d) nuclei form factor, (e) NE invaginations, and (f) heterochromatin spots in dHL60 neutrophils. Data points (red circles) pooled from three independent experiments are presented as mean ± s.e.m., with multiplicity-adjusted P values from ordinary one-way ANOVA. g. Scatter plot (left) and histogram (right) showing the gating strategy used for the analysis of flow cytometry data plotted in Fig. 4e-g. Graphs are representative of DMSO-treated SCR dHL60. Source numerical data and unprocessed western blots are available in the source data file.
