Extended Data Fig. 6: LMNA KO dHL60 cells exhibit a proteome profile closer to PMNs and migrate better relative to SCR dHL60 cells.
From: Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation
a-b. Graphs showing the fold change in the gene ontology (GO, biological process) profile of the associated (a) downregulated and (b) upregulated proteins in LMNA KO dHL60 cells relative to SCR dHL60 cells, quantified using tandem mass tagging (TMT)-based mass spectroscopy analysis. c-f. Scatter dot plots showing (c) the number of cells migrating towards fMLF for 1 hr, (d) median speed, (e) median directionality, and (f) cell eccentricity. Data is plotted as mean ± s.e.m. of 9, 8, and 5 independent experiments (red circles) for SCR, LMNA KO, and LMNA/LBR KO samples, respectively. For cell eccentricity, a total of 29, 45, and 16 data points for SCR, LMNA KO, and LMNA/LBR KO, were pooled from three independent experiments. The multiplicity-adjusted P values calculated using mixed-effect analysis (c-e) and ordinary one-way ANOVA (f) are shown. Source numerical data is available in the source data file.
