Extended Data Fig. 9: PLA of exosomes and secreted DNA in TPA-treated ears.
From: Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation
a. Schematic illustrating the antibody binding sites on FLAP and the principle of the PLA used to assess the presence of SEADs in inflamed ears. b. Airyscan microscopy images of ear cryosections (20 μm) from mice treated with TPA for 12 hrs, showing the status of PLA dots (magenta) in either FLAP- or dsDNA-only antibody conditions, used to test the nonspecific PLA signal. Sections were co-stained with DAPI (nucleus). Presented ‘sum of slices’ is representative of three independent experiments, and Scale is 100 μm. c. Airyscan microscopy images of ear cryosections (20 μm) from a mouse injected with either PBS or rDNase I and treated with TPA for 12 h, showing the status of CD63-citH3 PLA dots (magenta) in indicated conditions. Sections were co-stained with DAPI (nucleus). Zoomed insets are presented as inverted grayscale above respective conditions. Presented ‘sum of slices’ is representative of two independent experiments, and scale is 100 μm, in the inset it is 20 µm.
