Extended Data Fig. 2: NE-derived exosomes are spatially associated with secreted nuclear DNA but not migrasomes.
From: Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica Stellaris-5 cryo-confocal, showing the reflected light (grey) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. Representative of two biological replicates. The scale bar is 10 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOXgreen fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing bead-on-string-like structures (yellow arrowheads). The scale bar is 100 nm, and in the inset, it is 10 nm. d. Airyscan microscopy image of Hoechst 33342 (grey) stained PMN showing the extracellular distribution (white inset, zoomed below) of TSPAN4 (magenta) and 5LO (cyan). Cell is outlined in yellow, and scale is 5 µm, in the inset it is 1 µm. Representative of two biological replicates.
