Fig. 7: Cytoplasmic dilution-driven changes in mitotic architecture in early-differentiated cells.
From: Cell state-specific cytoplasmic density controls spindle architecture and scaling
a, For both cell states (ESCs, yellow; DIF, blue), number of astral microtubules is estimated from spindle volume data (experimental data as Fig. 1h and Supplementary Note Fig. 3) and grouped by cell volume bins of 500 µm3. Large circles depict averages for each cell volume bin (modelled data) and are fitted to equation (6), error bars show s.d. Solid curves represent fit curves. Dashed lines depict predicted saturation values for astral microtubule number. b, Upon differentiating of ESCs, a reduction in cellular mass density leads to enlargement of the pericentriolar material (PCM), increasing centrosomal nucleation capacity and redistribution of microtubule mass away from the spindle bulk towards the asters.
