Extended Data Fig. 3: LRNs in meT7 nuclei.
From: Crossover patterning through condensation and coarsening of pro-crossover factors
a, Schematic of a late pachytene nucleus in a hermaphrodite homozygous for the III; X; IV triple fusion chromosome meT7. Paired axes are shown in magenta to correspond to HTP-3 immunofluorescence shown in panel b. The meT7 chromosome is marked by HIM-8 (red), which binds the pairing center region on the normal X chromosomes. Late recombination nodules (LRNs) are marked by COSA-1. b, Representative immunofluorescence images showing late pachytene nuclei with paired meT7 fusion chromosomes. GFP::COSA-1 intensity at LRNs within these nuclei is heterogeneous, in contrast to wild-type oocytes. Scale bar, 5 µm. c-f, Quantification of GFP::COSA-1 and ZHP-3::GFP fluorescence intensities at individual LRNs in meT7 nuclei at late pachytene. Each dot represents the summed intensity for one focus. Lines connect values for foci from the same nucleus. The foci associated with meT7 foci were identified by HIM-8 and HTP-3 staining. The ‘non-meT7’ foci are on the normal autosomes, shown in an arbitrary order (these chromosomes were not identified individually). Intensities were normalized such that the average value of foci on non-meT7 chromosomes was 1. For nuclei with 5 COSA-1 or ZHP-3 foci, the brighter foci on meT7 were mandatorily assigned to the first column. n = 46, 40, 53, and 44 nuclei, from 6, 6, 16, and 16 animals, for nuclei with 4 GFP::COSA-1 foci, nuclei with 5 GFP::COSA-1 foci, nuclei with 4 ZHP-3::GFP foci, and nuclei with 5 ZHP-3::GFP foci, respectively, pooled from two independent replicates. See Fig. 2 for detailed comparisons. Source numerical data are available in source data.
