Extended Data Fig. 9: DAPI-staining bodies and LRN formation in various phosphosite mutants.
From: Crossover patterning through condensation and coarsening of pro-crossover factors
a-b, Graphs showing the distribution of nuclei containing varying numbers of DAPI-staining bodies (bivalents and univalents) at diakinesis in various phosphosite mutants at 15°C (a) and 20°C (b). In a, n = 147, 67, 140, 138, 139, and 153 scored nuclei from 39, 21, 38, 36, 37, and 39 animals, for wild type, msh-5 13A, zhp-3 4A, zhp-4 2A, zhp-3 4A; zhp-4 2A, and zhp-3 4A; msh-5 13A; zhp-4 2A, respectively, pooled from two independent replicates. In b, n = 193, 127, 158, 153, 151, and 159 scored nuclei from 39, 31, 36, 34, 35, and 41 animals, for wild type, msh-5 13A, zhp-3 4A, zhp-4 2A, zhp-3 4A; zhp-4 2A, and zhp-3 4A; msh-5 13A; zhp-4 2A, respectively, pooled from two independent replicates. One-way ANOVA and post hoc pairwise comparisons using t-tests with pooled s.d.. P value adjustment method: Holm. Comparisons between each genotype in a, not significant. Triple mutants vs. wild type or msh-5 13A in b, P = 0.0051 or 0.0147. Other comparisons in b, not significant. c, Representative immunofluorescence images showing GFP::COSA-1 staining from mid-pachytene to diplotene onset in various phospho-mutants at 25 °C. SC was marketed by SYP-1 staining. Scale bar, 5 μm. Source numerical data are available in source data.
