Extended Data Fig. 1: Dynamics of SC components and the pro-CO proteins.
From: Crossover patterning through condensation and coarsening of pro-crossover factors
a, Diffusion coefficients derived from autocorrelation functions (ACFs) for individual voxels in confocal line scans for each protein. Black boxes and bars indicate the average and median values, respectively. Number of voxels: n = 152 (HIS-72::GFP, LP), 104 (HTP-3::GFP, LP), 80 (GFP::HIM-3, LP), 104 (SYP-1::GFP, LP), 56 (SYP-2::GFP, LP), 112 (GFP::SYP-3, EP), 120 (GFP::SYP-3, LP), 104 (ZHP-3::GFP, SC, mid-pachytene), and 112 (ZHP-3::GFP, LRN, late pachytene), respectively, pooled from two independent replicates. Voxels are from the line scans in Fig. 1c. See Fig. 1c for the number of scans for each genotype or condition. One scan per SC. Two-tailed Mann-Whitney U test. Detailed statistical comparisons (including P-values) are reported in Supplementary Table 2 (red). SC, synaptonemal complex, EP, early pachytene. LP, late pachytene. LRN, late recombination nodule. Protein names are colorized based on the structures that they associate with (chromatin, chromosome axis, SC, and/or RNs). b, Movement indices based on paired correlation functions (pCFs) of single voxel pairs. Black boxes and bars indicate the average and median, respectively. Number of voxel pairs: n = 91 (SYP-1::GFP, LP), 49 (SYP-2::GFP, LP), 105 (GFP::SYP-3, EP), 105 (GFP::SYP-3, LP), 91 (ZHP-3::GFP, SC, mid-pachytene), and 91 (ZHP-3::GFP, LRN, late pachytene), respectively, pooled from two independent replicates. Voxel pairs are from the line scans in Fig. 1d. See Fig. 1d for the number of scans for each genotype or condition. One scan per SC. Two-tailed Mann-Whitney U test. Detailed statistical comparisons (including P-values) are reported in Supplementary Table 3 (red). c, Representative FRAP analysis of ZHP-3::GFP along SCs in a mid-pachytene nucleus. The green arrow indicates the bleached region. ZHP-3::GFP intensity recovered within minutes after photobleaching, while HTP-3 (see Extended Data Fig. 2, performed in parallel) showed no recovery. Elapsed time is expressed in minutes:seconds relative to the moment of bleaching. Scale bar, 5 µm. d, FRAP quantification. The average intensities of the regions of interest over time were normalized against their average intensity before photobleaching. Means ± s.e.m. were plotted. n = 12 nuclei from 7 animals for each condition, pooled from two independent replicates. For each nucleus, a bleached region and a similar unbleached (control) region in the same nucleus were measured. Source numerical data are available in source data.
