Extended Data Fig. 2: SC confines the movement of its components within individual SCs.
From: Crossover patterning through condensation and coarsening of pro-crossover factors
a, Representative time-lapse images showing fluorescence recovery after photobleaching (FRAP) of GFP::SYP-3 along a segment of an SC in a mid-pachytene nucleus. The green arrow indicates the photobleached region. Time is indicated in minutes:seconds relative to the moment of bleaching. b, Quantification of GFP::SYP-3 intensity over time as described in panel a. In each nucleus, a bleached region and a similar unbleached control region were measured. Intensities were normalized based on prebleached values in the region of interest. Time is indicated in minutes after bleaching. n = 15 nuclei from 6 animals for each condition, pooled from two independent replicates. c, Representative time-lapse images showing FRAP of GFP::SYP-3 following bleaching of an entire SC in a mid-pachytene nucleus. The green box indicates the photobleached region. d, Quantification of GFP::SYP-3 intensity over time as described in c. n = 14 nuclei from 6 animals for each condition, pooled from two independent replicates. e, Representative images showing FRAP of HTP-3::GFP along a segment of paired chromosome axes in a mid-pachytene nucleus. The green arrow indicates the photobleached region. f, Quantification of HTP-3::GFP intensity over time as described in panel e. n = 12 nuclei from 5 animals for each condition, pooled from two independent replicates. g, FRAP of HTP-3::GFP on an entire chromosome pair in a mid-pachytene nucleus. The green box indicates the photobleached region. h, Quantification of HTP-3::GFP intensity over time as described in panel g. n = 12 nuclei from 5 animals for each condition, pooled from two independent replicates. Scale bars in a, c, e and g: 5 μm. Means ± s.e.m. were plotted in b, d, f, h. Source numerical data are available in source data.
