Fig. 2: Impact of vha-6, vha-8, vha-14, vha-15, vha-20, vha-16 and vha-19 RNAi on gene expression and lifespan of C. elegans.
From: A lysosomal surveillance response to stress extends healthspan
a, A qRT–PCR analysis of transcripts (n = 4 biologically independent samples) in worms treated with control (ev) or RNAi targeting v-ATPase subunits (****P < 0.0001). b,c, The GFP–CPR-5 expression level (b) and survival (c) of worms treated with control or 10–60% vha-6 RNAi. The control RNAi was used to supply to a final 100% of RNAi for all conditions (****P < 0.0001). d, A schematic diagram showing the regions on mRNA targeted by the three vha-6 RNAi obtained from either the Vidal (vha-6_1) or Ahringer (vha-6_2, vha-6_3) library. CDS, coding sequence. e,f, The GFP–CPR-5 expression level (e) and survival (f) of worms treated with control or the vha-6 (20%) RNAi as indicated in d (****P < 0.0001). g–l, vha-6 RNAi extends the lifespan of daf-2(e1370) (g), daf-16(mu86) (h), raga-1(ok386) (i), aak-2(ok524) (j), eat-2(ad465) (k) and atfs-1(gk3094) (l) mutants by 61%, 65%, 55%, 132%, 26% and 64%, respectively (****P < 0.0001). Scale bars, 0.3 mm. The error bars denote the standard error of the mean. The statistical analysis was performed by ANOVA followed by Tukey’s post hoc test in a or a log-rank test in c and f–l. The statistical data for lifespan can be found in Supplementary Table 1.
