Extended Data Fig. 1: Lifespan and gene expression changes in response to RNAi targeting different v-ATPase subunits in C. elegans.
From: A lysosomal surveillance response to stress extends healthspan
a-m, Survival of worms treated with control (ev), or RNAi targeting different v-ATPase subunits. Each v-ATPase RNAi occupied 40%, except for vha-11 and vha-12 RNAi, which occupied 10%; control RNAi was used to supply to a final 100% of RNAi for all conditions. The percentages indicate the mean lifespan changes as compared to the control condition (****P < 0.0001; in (b), **P = 0.0015; in (e), **P = 0.0056; in (f), P = 0.0580 (N.S.); in (h), P > 0.9999 (N.S.); in (m), P = 0.7280 (N.S.)). n-s, Transgenic worm strains expressing mCherry tagged VHA-6 (n), and GFP-tagged VHA-14 (o), VHA-15 (p), VHA-16 (q), VHA-20 (r) and VHA-1 (s), were treated with the corresponding VHA RNAi and examined for fluorescence intensity. t, Functional clustering of the 3,322 differentially expressed genes (DEGs) that commonly up-regulated in in response to vha-6, vha-16 and vha-19 RNAi (25%). P. Value was derived from DAVID (one-sided Fisher’s Exact test). u, GFP expression levels of cpr-5p::gfp worms treated with RNAi targeting different v-ATPase subunits. v, vha-6 RNAi (100%) extended wild-type N2 worm lifespan by 26%, even when RNAi treatment started since the L4/young adult stage (****P < 0.0001). Scale bars, 0.3 mm. Statistical analysis was performed by log-rank test (N.S., not significant). Statistical data for lifespan can be found in Supplementary Table 1.
