Extended Data Fig. 8: BMP4 induction and Immunosuppressive factors expression in amnion.
From: Atlas of amnion development during the first trimester of human pregnancy
(a) In vitro stem cell experiment design (left) and qPCR results (right) of cytokines-treated iPSCs. Relative gene expression was calculated using the ΔΔCt method, with GAPDH as the internal control. Each data point represents an individual sample, and bars indicate the mean expression value. The untreated medium was used as the control group, while groups treated with BMP4, RA, and MDK were the experimental groups. Each group, except for the BMP4-treated group, has two biological replicates. (b, c) Circle plots showing MIF (b) and SPP1 (c) interactions across different cell types. Analyses are generated from human amnion samples of the following developmental stages: CS16 (n = 1), CS17 (n = 1), CS19 (n = 1), CS22 (n = 1). (d) Immunostaining showing the expression of SPP1, MIF and CD44 in the CS16 amnion section, Scale bar = 50 μm. Representative image from 3 independent experiments.
