Extended Data Fig. 9: Replicate clustering and analysis of H3K9me2 Cut & Run in EpiLCs.
From: The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate
(a) Average genome tracks of Lamin B1 and LAP2β CUT & RUN in WT EpiLCs. (b) Overlap between LB1 and LAP2β domain loci in WT EpiLCs. (c) Dendrogram and heatmap of individual H3K9me2 Cut & Run replicates (3 per condition) showing similarity of replicates for each genotype. (d) Kernel density plot of spike-in normalized H3K9me2 signal intensity (RPKM) across genotypes. (e) Average genome tracks (n=3 replicates) and domain calls for H3K9me2 in WT, LBR KO, TKO, and QKO ESCs on a 12 Mb section of chromosome 12. Y-axis range indicated at top left is the same for all tracks shown. (f) Size of H3K9me2 domains in each genotype. (g) Number of H3K9me2 domains called in each genotype. (h) Standard deviation of H3K9me2 signal in WT EpiLC domains.
