Extended Data Fig. 2: Proliferation and pluripotency assays of iPS cell lines.
From: A programmed decline in ribosome levels governs human early neurodevelopment
a Growth of parental and AIRIMV190G lines. Error bars are SEM. b. Teratoma assay of SCVI274 (control) and the derived AIRIMV190G mutant (V190G) lines. Scale bar, 200 μm. c. Growth of parental and AIRIMR72W lines. Error bars are SEM. d. Immunofluorescence image of pluripotency markers SOX2 (green) and OCT4 (red) of AIRIMR72W iPS cell clones. Scale bar, 1 mm. e. Growth of parental and AFG2B patient lines. Error bars are SEM. f. Immunofluorescence image of pluripotency markers SOX2 (green) and OCT4 (red) of iPS cell clones derived from the AFG2B patient and parental control fibroblasts. Scale bar, 1 mm. Two-way RM ANOVA. n = 4 individual wells quantified per genotype, per time point for panels a,c,e.
