Extended Data Fig. 4: Additional AIRIMV190G variant clone derived from H9 cells exhibits similar growth defect as other 55LCC complex members.
From: A programmed decline in ribosome levels governs human early neurodevelopment
a Schematic illustrating the main stages of the method for generating human dorsal forebrain organoids75 from H9 hESCs. b. Sanger sequencing of C1orf109 V190G mutation in mutation cell line. c. Bright-field images of brain organoid at day 9 and day 20, with 2500 WT and V190G start cells respectively. d. Bright-field images of brain organoid at day 9 and day 20, with 9000 WT and V190G start cells, respectively. e. Measuring area of brain organoid generated from 2,500 WT and V190G cells (day 9 P = 0.047, day 20 P = 0.385, n = 3 biological replicates, unpaired, 2-tailed Student’s t-test), respectively. Data are presented as mean ± SD. f. Measuring area of brain organoid generated from 9,000 WT and V190G cells (day 9 P = 0.003, day 20 P = 0.150, n = 3 biological replicates, unpaired, 2-tailed Student’s t-test), respectively. Data are presented as mean ± SD.
