Extended Data Fig. 5: AIRIM variant organoids exhibit increased cytoplasmic RSL24D1.
From: A programmed decline in ribosome levels governs human early neurodevelopment
Polysome profiles of Control and AIRIMV190G a. iPS cells and b. day 15 organoids. Western blots for RSL24D1 and RPL28 of the fractions corresponding to the indicated regions of the gradient for Control and AIRIMV190G c. iPS cells and d. day 15 organoids. 3 independent batches were performed. e. IF showing the subcellular distribution of RSL24D1 in control and AIRIMR72W day 15 organoids. Scale bar, 20μm. f. Quantification of the nuclear/cytoplasm ratio of RSL24D1 immunofluorescence of day 15 control (WT) and mutant (V190G, R72W) iPS cells. *P(V190G vs Control) = 0.0302, *P(R72W vs Control) = 0.01. Kruskal-Walli’s comparison test (two-sided). n = 6 control and 5 mutant (V190G), n = 6 control and 6 mutant (R72W) imaged regions from 2 independent batches. Error bars are SEM. g. IF showing the subcellular distribution of RSL24D1 in parental and AFG2B variant day 15 organoids. Scale bar, 20μm. h. Quantification of the nuclear/cytoplasm ratio of RSL24D1 immunofluorescence of individual neuroepithelial bud of day 15 Parental and AFG2B variant organoids. **P = 0.0013, Unpaired two-sided test with Welch’s correction. n = 10 parental and 10 AFG2B variant imaged regions from 2 independent batches. error bars are SEM.
