Fig. 5: scRNA-seq reveals AIRIMV190G mutant organoids display a transient delay of neuroepithelial differentiation.
From: A programmed decline in ribosome levels governs human early neurodevelopment
a, UMAP embedding of scRNA-seq data from one pooled sample per condition (n = 50 day 5 EBs, 3–6 day 10 NE, 3–6 day 15 organoids and 3 day 30 organoids per genotype, 10,000 cells were sequenced per condition). tNE, transitioning NE; IP, intermediate progenitor; N, neuron; CP, choroid plexus. b, Split-view of full integrated dataset, coloured by Louvain clusters. c, Heatmap showing the average expression of cluster marker genes related to brain development from the full integrated dataset. d, Quantification of different cell types identified at each time point in control and V190G samples. e, Heatmap showing the average expression of translation regulator genes across the development of control and V190G organoids. f, Gene module expression scores for a set of all 79 human r-protein genes across developmental time points.
