Extended Data Fig. 6: Metabolic and lipidomic profile of human HSPCs upon aging.

a, Experimental design to characterize the metabolome and transcriptome of young and aged human HSPCs. The input material per sample is indicated. b, PCA of young and aged HSPCs RNA-seq samples based on the top 500 most variable genes. n = 4–7. c, Volcano plot of transcriptome DEGs in young vs aged HSPCs. P-adjusted value. NS, not significant. d, Heatmap representing relative expression of differentially expressed KEGG enzymes in young vs aged HSPCs (log2FC threshold = 1, p-adjusted < 0.05), classified in their respective metabolic pathways. e, GSEA of selected GO processes on RNA-seq of aged vs young HSPCs. P-adjusted value. f, GSEA of selected metabolic pathways on RNA-seq of aged vs young HSPCs. P-adjusted value. g, Pathway activity score estimated by decoupleR in young vs aged HSPCs RNA-seq. Differentially activated pathways are shown (p-value < 0.05). h, Heatmap of all detected metabolites passing quality thresholds in human targeted metabolomics of aged and young HSPCs. The log2FC values between each aged biological replicate and the average of young HSPCs are depicted. n = 14–17. ND, not detected. (b, h) n indicates the number of biological replicates per condition. For (h), 13 independent experiments were performed.