Fig. 2: Lipid composition of human HSCs and their progenitors.

a, The workflow of the method and analysis of low-input untargeted lipidomics in human HSPCs and progenitors. Created with BioRender.com. b, A pie chart depicting the number of detected lipids per class, with the total number per class indicated in square brackets. c, Lipheat depicting the top 30 detected lipids in HSPCs or progenitors ranked by log₂FC value. n = 14. d, Left: dot plots depicting the total number of carbons (length) and double bonds (unsaturations) in the acyl chains of detected lipids, categorized by class. Colour shows the log₂FC abundance in HSPCs versus progenitors. The dot size corresponds to the mean signal intensity of the detected peaks per lipid across all biological replicates. Right: bar plots of the summed signal intensity of lipids per class in HSPCs and progenitors. Subclasses were based on unsaturation levels (saturated (0 unsaturation), monounsaturated (1 unsaturation) and polyunsaturated (≥2 unsaturations)) or acyl-chain length (short (<36 carbons) and long (≥36 carbons)). Significantly different abundances per class and subclass are shown. Paired two-sided Student’s t-test or Wilcoxon test. e, A volcano plot of differentially abundant detected metabolites and lipids between HSPCs and progenitors. P-adjusted value after paired Student’s t-test or Wilcoxon test. NS, not significant. In c, n indicates the number of biological replicates per condition. For b–d, eight independent experiments were performed. Iso, isopropanol; CAR, carnitines; LPC, lyso-PC; PI, phosphatidylinositol; TG, triacylglyceride.