Fig. 3: Metabolic and lipidomic profile of mouse HSCs and progenitors.

a, The experimental design to characterize the metabolome of mouse HSCs and progenitors. The input material per sample, number and type of detected metabolites and genes are indicated. b, A schematic summary of metabolomics results per pathway in mouse HSCs and progenitors. Quantification bar plots as mean ± standard deviation show raw values per biological replicate. Paired samples are linked by grey lines. Differentially abundant metabolites are depicted as coloured dots in the scheme. n = 13. P-adjusted value after paired two-sided Student’s t-test or Wilcoxon test is shown. c, The experimental design to characterize the lipidome of mouse HSCs and progenitors. The input material per sample, number and type of detected lipids are indicated. d, Bar plots of the summed signal intensity of lipids per class in HSCs and progenitors. Subclasses were based on unsaturation levels or acyl-chain length. Significantly different abundances per class and subclass are shown. Paired two-sided Student’s t-test or Wilcoxon test. e, Lipheat depicting the detected lipids in HSCs versus progenitors ranked by log₂FC value. n = 10. In b and d, n indicates the number of biological replicates per condition. For b, four independent experiments were performed. For d and e, three independent experiments were performed. Panels a and c created with BioRender.com.