Extended Data Fig. 2: Metabolic profile of human BM HSPCs and their downstream progenitors.

a, Pearson correlation between enzyme expression and metabolite abundance per sample in selected KEGG pathways. Color and size of the dots represent the correlation coefficients. n = 4. Correlation p-value; *p < 0.05. b, Metabolograms integrating metabolomics and transcriptomics data on selected KEGG pathways. Color represents the log2FC between HSPCs and progenitors. Inner circles indicate the average tendencies, and outer circles show individual genes/metabolites. P-adjusted value; *p < 0.05, **p < 0.01, ***p < 0.001. c, Subnetwork representing the COSMOS mechanistic hypotheses of metabolic/transcriptomic regulation of multiple connected pathways, based on HSPCs vs progenitors metabolomics and RNA-seq datasets. Each node represents a gene or metabolite. The border color depicts the estimated activity by the model. Arrow shape corresponds to the type of regulation based on ground knowledge. The fill color of elements shows their level of differential expression or abundance in our measured data. P-adjusted value; *p < 0.05, **p < 0.01, ***p < 0.001. (a) n indicates the number of biological replicates per condition.