Extended Data Fig. 6: Assessment of PEX7 complex formation and confidence measures of predicted AlphaFold modelling.
From: PEX39 facilitates the peroxisomal import of PTS2-containing proteins
a, Native-PAGE and Coomassie/TCA staining of the indicated recombinant proteins. In-gel positions of PEX7 alone (PEX7), PEX5 alone (PEX5), and the PEX7-PEX5-PHYH complex (*) are indicated. H6PHYH is a basic protein that does not enter these gels alone33. b, Confidence measures for human protein complex predictions. Predicted aligned error plots and predicted LDDT (local distance difference test) plots of human AlphaFold models. To predict human protein complexes, PEX39 (full-length), PEX7 (full-length), the PTS2 sequence of PHYH (amino acids 1-24), and the PEX7 binding domain of the long isoform of PEX5 (amino acids 179-266) were used. c, Confidence measures for yeast protein complex predictions. Same plots as described in b for predictions of yeast protein complexes. Protein complexes from yeast were predicted using ScPex39 (full-length), Pex7 (full-length), the PTS2 sequence of Pot1 (amino acids 1-30), and the Pex7 binding domain of Pex18 (amino acids 192-283).
