Extended Data Fig. 9: Characterization of ScPex13 with a mutated KPWE motif.
From: PEX39 facilitates the peroxisomal import of PTS2-containing proteins
a, Biological replicates of the cellular fractionation of yeast expressing the ScPex13 KPWE-to-AAAA mutant. Experiments were performed on the indicated strains as described in Fig. 6g. b, Characterization of organelle-associated Pex7 and Pot1 in yeast expressing the ScPex13 KPWE-to-AAAA mutant. The strains used are described in Fig. 6g. Top: immunoblots of cellular fractionation of yeast expressing the ScPex13 KPWE-to-AAAA mutant as described for Fig. 6g. Bottom: immunoblot analysis of salt-extraction of proteins from organellar pellets isolated as described for Fig. 6g. T, total; S, supernatant fraction; P, pellet fraction.
