Fig. 7: Distinct hierarchy of assembly between WIPI-driven mitophagy and FIP200-driven mitophagy or starvation-induced autophagy.
a, Immunoblotting for phosphorylated SQSTM1/p62 in WT or ATG13 KO cells (clone #1), where indicated rescued with ATG13 WT or ATG13 lacking residues 190–230 (Δ190–230). Data are presented as mean ± s.d. (n = 3 biologically independent experiments). One-way ANOVA with Dunnett’s multiple comparisons test. b, Immunoblotting for LC3B in the same cell lines as used in a, but treated with 2-h Earle’s balanced salt solution (EBSS) starvation medium and bafilomycin A1 (BafA1) where indicated. Data are presented as mean ± s.d. (n = 3 biologically independent experiments). Tukey’s multiple comparisons test. c, Mitophagy flux measured by flow cytometry of WT or ATG13 KO HeLa cells, overexpressing BFP-Parkin, where indicated rescued with ATG13 WT or ATG13 lacking residues 190–230 (Δ190–230), and left untreated or treated with O/A for 5 h. Data are presented as mean ± s.d. (n = 3 biologically independent experiments). Two-way ANOVA with Tukey’s multiple comparisons test. d, As in c, but with WT HeLa cells, overexpressing BFP-Parkin, transfected with siRNAs targeting ATG13, left untreated or treated with O/A for 5 h. Data are presented as mean ± s.d. (n = 3 biologically independent experiments). Two-way ANOVA with Tukey’s multiple comparisons test. e, Immunoblotting of COXII levels in WT or ATG13 KO HeLa cells, overexpressing BFP-Parkin, and where indicated rescued with ATG13 WT or ATG13 lacking residues 190–230 (Δ190–230), left untreated or treated with O/A for 24 h. Data are presented as mean ± s.d. (n = 3 biologically independent experiments). One-way ANOVA with Dunnett’s multiple comparisons test. *P < 0.05, ***P < 0.001, ****P < 0.0001. NS, not significant. Source numerical data, including exact P values, are available in the source data.
