Extended Data Fig. 4: Monitoring mitophagy in ATG7 KO MEF expressing the ER-phagy receptors IDR modules. | Nature Cell Biology

Extended Data Fig. 4: Monitoring mitophagy in ATG7 KO MEF expressing the ER-phagy receptors IDR modules.

From: The intrinsically disordered regions of organellophagy receptors are interchangeable and control organelle fragmentation, ER-phagy and mitophagy flux

Extended Data Fig. 4

ATG7KO MEF were exposed to BafA1 to preserve mitochondria (labeled with TOMM20, red) possibly transported within LAMP1-positive degradative endolysosomes (cyan) and imaged in CLSM. The absence of mitochondria in the lumen of the endolysosomes certifies absence of detectable mitophagy. Scale bar: 10 µm, inset magnification: 4x. (b) Same as the CLSM image in (a) for cells expressing Mito-GFP at the OMM. (c) same as (b) for cells expressing Mito-GSEC62IDR. (d) Same as (c) for cells expressing Mito-GSEC62IDRLIR. (e) same as (c) for Mito-GFAM134BIDR. (f) Same as (d) for Mito-GFAM134BIDRLIR. (g-h) Same as (c-d) for Mito-GTEX264IDR and Mito-GTEX264IDRLIR. (i) LysoQuant quantification of the percentage of cellular endolysosomes that are degrading TOMM20-positive mitochondria fragments in panels A-H (N = 3 biological replicates for a-d, N = 1 for e-h, n = 31, 19, 21, 29, 37, 26, 28, and 29 cells for a-h, respectively). Ordinary one-way ANOVA with Turkey’s multiple comparisons test, F = 1.4. Mean bar is shown. Adjusted P value: ns (P = 0.2070), not significant.

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