Extended Data Fig. 1: Specification of trophectoderm (32-TE) and inner cell mass lineages occurs at the 32-cell stage and the gene transcription profile of 32-TE is distinct from that of classic trophoblast stem cells. | Nature Cell Biology

Extended Data Fig. 1: Specification of trophectoderm (32-TE) and inner cell mass lineages occurs at the 32-cell stage and the gene transcription profile of 32-TE is distinct from that of classic trophoblast stem cells.

From: Mouse trophectoderm stem cells generated with morula signalling inducers capture an early trophectoderm state

Extended Data Fig. 1

(a) 16-cell and 32-cell stage embryos stained with PKH26 followed by dissociation. Manually selected dissociated outer blastomeres are shown. (b) ESCs (GFP positive) integrate into the ICM of blastocysts upon aggregation or injection. (c) Aggregation of 32-cell outer blastomeres (10 or 20) with 5 ESCs resulted in the formation of blastoids. (d) Degenerated decidua induced by blastoids after implantation on the 6th day. (e) Percentage of aggregates formed by different combinations of inner and outer blastomeres in 32-cell stage embryos (related to Fig. 1c). (f) Percentage of live births after blastocyst (control) or aggregates transferred to the mouse uterus (related to Fig. 1c). (g) Percentage of decidualization and live births after blastocyst (control) or blastoids transferred to the mouse uterus. (h) Heatmap analysis of differentially expressed genes between 32-TE cells and TSCs. 32-TE, outer cells of 32-cell stage embryos. (i) Heatmap analysis of differentially expressed genes between 32-TE, E3.5, TESCs, and TSCs. Differentially expressed genes were those with Padj<0.05 and log2FoldChange ≥ 1 in transcriptome sequencing analysis. Experiments were repeated three times (a-d) with similar results. Data are shown as mean ± SEM from three independent experiments (e-g). Numerical data are available as source data. Scale bars: 50 μm in a-c; 5 mm in d.

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