Extended Data Fig. 8: Menin and SUZ12 inhibition accelerates CM differentiation.
From: Genome-wide CRISPR screen identifies Menin and SUZ12 as regulators of human developmental timing
a, mRNA expression of representative CM markers, relative to housekeeping gene, GAPDH, assessed by qRT-PCR (n = 3 independent differentiations). Upper panels, Time course analysis in the vehicle control cells. Data are each replicate with means connected. Bottom panels, Quantification of CM markers at the onset of gene upregulation or downregulation following inhibitor treatment. P values were calculated using one-way ANOVA test with post-hoc Dunnett’s multiple comparisons test. P values were indicated on the plots unless not significant (ns, P > 0.05). b, Quantification of cTnT+ cell percentage at day 7 (n = 2 independent differentiations). c, Representative contour plot and histograms show bimodal distribution of cTnT expression level in day 7 cells, and the gating strategy to define cTnT+ cells. d, Mean fluorescent intensity (MFI) of cTnT in cTnT+ cells in day 7 and 9 cells (n = 2 independent differentiations).
