Fig. 2: Menin or SUZ12 inhibition accelerates expression of neuroectodermal genes.
From: Genome-wide CRISPR screen identifies Menin and SUZ12 as regulators of human developmental timing
a,b, PCA plots of the first set of RNA-seq experiment of NT and SUZ12 KO samples (a) and the second set of RNA-seq experiment of NT and MEN1 KO samples (b). Samples are distributed according to their day of differentiation based on top 500 differentially expressed transcript with variance stabilized normalization. c,d, Temporal expression patterns of monotonic up clusters 1 and 4 (c) and monotonic down clusters 7 and 8 (d) in the NT samples identified by TCseq. e,f, Z-score normalized expression of cluster 1 and 4 genes (e) and cluster 7 and 8 genes (f) in MEN1 or SUZ12 KO versus NT control are shown in the line graphs (n, number of genes in each cluster; c,d). Data are the mean ± s.e.m. Normalized counts of selected representative markers of cluster 1 and 4 (c) and cluster 7 and 8 (d) are shown in the bar graphs (n = 3 independent differentiations). Data are the mean ± s.d. P-values were calculated from two-way ANOVA test with post-hoc Tukey’s multiple comparisons test. g,h, GSEA enrichment plots of cluster 1 and 4 genes (g) and cluster 7 and 8 genes (h) in MEN1 or SUZ12 KO versus NT comparisons at day 4. FDR, false discovery rate; NES, normalized enrichment score.
