Extended Data Fig. 6: Adoptive transfer of CD160+CD8+ T cells inhibit the growth of MC38 subcutaneous tumor.
a, Schematic representation of co-transfer of CD160+CD8+ T cells (GFP+) and CD160−CD8+ T cells (CD45.1+) into MC38 tumor-bearing mice. b, Representative flow cytometry identification plots of splenocytes from CD45.1 and GFP mice. c, d, Representative flow cytometry plots (c) and frequencies (d) of GFP+CD45.1− and GFP−CD45.1+ in tumors 36 hours after transfer (n = 6 mice). e, f, Representative flow cytometry plots (e) and frequencies (f) of CD45.1+Tetramer+ cells in tumors at indicated timepoints after adoptive transfer CD160+CD8+ T cells from CD45.1 mouse spleens into established MC38-OVA tumors (n = 5 mice). g, h, Representative flow cytometry plots (g) and frequencies (h) of CD160 in CD8+ T cells isolated from tumor tissues, as shown in Fig. 4m (n = 5 mice). i, Representative flow cytometry plots of GzmB in CD8+ T cells isolated from tumor tissues, as shown in Fig. 4m. j, Experimental scheme for adoptive T cell transfer in established MC38 tumors. Splenic CD160+CD8+ and CD160−CD8+ T cells from normal syngeneic C57BL/6 mice were transferred via tail vein injection at the indicated time points. k, l, Representative tumor images (k) and tumor volume (l) in MC38 tumor mouse model, as shown in (j) (n = 5 mice). m, n, Representative flow cytometry plots (m) and frequencies (n) of CD160 in CD8+ T cells isolated from tumor tissues, as shown in (j) (n = 5 mice). o, p, Representative flow cytometry plots (o) and frequencies (p) of GzmB in CD8+ T cells isolated from tumor tissues in MC38 tumor-bearing mice, as shown in (j) (n = 5 mice). Data are shown as the mean ± s.e.m, P values were determined by unpaired two-tailed Student’s t-test (d, h, l, n and p).
