Extended Data Fig. 7: Effects of CD160 expression levels on CD8+ T cell anti-tumor function in MSS CRC model and validation of CD160 knockout efficiency of Cd160−/− mice.
a, Experimental scheme for adoptive T cell transfer while inoculating the CT26 cell line into Balb/c mice. Splenic CD160+CD8+ and CD160−CD8+ T cells from normal syngeneic Balb/c mice were transferred via tail vein injection at the indicated time points. b, c, Representative tumor images (b) and tumor volume (c) in CT26 tumor-bearing mice, as shown in (a) (n = 5 mice). d, Frequencies of CD160 in CD8+ T cells isolated from tumor tissues in CT26 tumor-bearing mice, as shown in (a) (n = 5 mice). e, Frequencies of GzmB in CD8+ T cells isolated from tumor tissues in CT26 tumor-bearing mice, as shown in (a) (n = 5 mice). f, Experimental scheme for adoptive T cell transfer in established CT26 tumors. Splenic CD160+CD8+ and CD160−CD8+ T cells from normal syngeneic Balb/c mice were transferred via tail vein injection at the indicated time points. g, h, Representative tumor images (g) and tumor volume (h) in CT26 tumor-bearing mice, as shown in (f) (n = 4 mice). i, Frequencies of CD160 in CD8+ T cells isolated from tumor tissues in CT26 tumor-bearing mice, as shown in (f) (n = 4 mice). j, Frequencies of GzmB in CD8+ T cells isolated from tumor tissues in CT26 tumor-bearing mice, as shown in (f) (n = 4 mice). k, Scheme of generation of Cd160−/− mice. l–n, Representative flow cytometry plots and frequencies of CD160 in CD8+ T cells isolated from spleen (l), small intestine (m) and colon (n) in WT and Cd160−/− mice. o, p, Representative flow cytometry plots (o) and frequencies (p) of CD160 in CD8+ T cells isolated from tumor tissues in WT and Cd160−/− mice bearing MC38 tumor, as shown in Fig. 5e (n = 6 mice). Data are shown as the mean ± s.e.m, P values were determined by unpaired two-tailed Student’s t-test (c, d, e, h, i, j, l, m, n and p).
