Extended Data Fig. 10: Representative gating strategies for T lymphocytes.

a, FACS gating strategy of CD160⁺ in CD8⁺ T cells isolated from intestine and spleen. Related to Fig. 2k; Extended Data Fig. 4e, i; Extended Data Fig. 7l, m, n. b, FACS gating strategy for in vitro exhaustion assay. Related to Fig. 4d. c, FACS gating strategy for co-culture system. Related to Fig. 4g. d, FACS gating strategy of CD160⁺ and GzmB⁺ cells in tumor-infiltrating CD8⁺ T cells. Related to Fig. 5h; Extended Data Fig. 6g, i, m, o; Extended Data Fig. 7o; Extended Data Fig. 8a, c, m; Extended Data Fig. 9h, k. e, FACS gating strategy of CD160⁺CD8⁺ T cells (GFP⁺CD45.1⁻) and CD160⁻CD8⁺ T cells (GFP⁻CD45.1⁺) int tumor. Related to Extended Data Fig. 6c. f, FACS gating strategy of CD45.1⁺Tetramer⁺ cells in tumor. Related to Extended Data Fig. 6e. g, FACS gating strategy of progenitor exhausted CD8⁺ T cells in tumor. Related to Fig. 5l; Extended Data Fig. 8d, n. h, FACS gating strategy of FcεR1γ⁺ in CD160⁻CD8⁺ T cells or CD160⁺CD8⁺ T cells. Related to Fig. 7e. i, FACS gating strategy of 4-1BB⁺ in CD160⁻CD8⁺ T cells or CD160⁺CD8⁺ T cells. Related to Fig. 7i. j, FACS gating strategy of GzmB⁺ in CD160⁻CD8⁺ T cells or CD160⁺CD8⁺ T cells. Related to Fig. 4a; Fig. 7f, g, j, k. k, FACS sorting strategy of CD160⁺CD8⁺ T cells from spleen and purity validation.