Extended Data Fig. 3: Analyses of transcriptional and chromatin changes in single cells in response to mechano-osmotic stimuli.
From: Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells
(a) Quantification of open chromatin from scATACseq of hiPSCs compressed (Comp) for 5 or 30 mins with or without 24 h recovery (Rec) as well as uncompressed controls (CNL). Note reduced accessibility at transcription start sites (TSS) at 30 min compression. (b) Violin plots of computed apoptosis, necrosis, starvation and hypoxia scores from single cell RNAseq across all conditions. (c) UMAP of cell cycle stage distribution from single cell RNAseq across all conditions. (d) Heatmap of ChromVAR analysis from scATACseq of hiPSCs compressed for 30 mins with or without 24 h recovery. (e) Volcano plot of differential gene expression between 30 min compression and CNL. (f) Violin plots of POU5F1 and SOX2 gene expression across all conditions (Wald/IHW).
